Mr. Chetan Tapadia .
Relique Technologies
35/D, Senadatta Peth
Pune 411 030
Subject : Efficacy test for sterile air
Dear sir,
The procedure for testing :
Two sets of samples were collected for checking sterility efficacy of the apparatus provided. Lab space 20 x 20 ft was used for the experiment. Steromax air sterilizer was used to produce sterile air in the above room for two different time intervals: 2 hrs and 18 hrs. Sterile nutrients plates (Malt extract yeasty extract glucose peptone agar and Nutrient agar) were exposed for 15 min before and after test period. Number of colony forming units (CFU) were noted after incubation of plates after 72 h at room temperature.. Experiment was carried out in duplicates.
Set 1: Microbiological count before and after operation of Steromax air sterilizer for 18 h
(without human interference)
Medium used
CFC before sterilization
CFC after 18 h sterilization by using Steromax
MGYP
15
6
NA
14
8
Set 2: Microbiological count before and after operation of Steromax air sterilizer for 2 h
(with human interference)
Report on testing of one U.V. based air purifier, Brand: "Sterlo-Max", submitted by M/s. Relique Technologies, Pune — 411 030, vide letter dated 06.09.04.
Sample :
Steel made U.V. Electrostatic precipitator based air sterilizer.
Brand: "Sterlo-Max". Model: SM2K.
Sr. No.: 0004000006.
Test Procedure:
Initially, the control count of bacterial and fungal organisms was ascertain from different location of nonsterile room of 3.5 X 3.2 X 3.2 m3. Size with one ventilation netted window . This is done by expose plate method i.e. by exposing open sterile nutrient agar plates to and fro, up and down in a one cubic meter area at a distance of half meter(near the blower of sterilizer and one meter from the location of sterilizer (at two different direction i.e. at center and right respectively) when it is in off condition(sterilizer was kept in front of the window at a height of two feet). Such a air sampling was done for 3 minutes, then the plates were closed and kept for incubation for 48 hours at 37 °C (plate sampling were done in duplicate).
This procedure were repeated while the above mentioned air sterilizer was in operation, at different time intervals (after air sterilizer was put on).
Same procedure were conducted with the sterile sabouraud's agar plates for ascertaining viable fungal counts, if any in the same room.
Results:
Bacterial Counts(CFU)
at different time
intervals
Bacterial CFU at
the center qf the
room near the blower
of sterilizer
(at a distance of
half mtr from sterilzer )
Bacterial CFU* at left side (at a distance of 1 mtr.from sterilizer)
Control counts (when the sterilizer was put off)
130
20
30 minutes.after sterilizer was put on
3
5
1 hour after sterilizer was put on
1
3
2 hours after sterilizer was put on
1
2
Control counts (When the sterilizer was put off)
17
21
30 minutes after sterilizer was put on
2
2
1 hour after sterilizer was put on
1
1
2 hour after sterilizer was put on
1
1
* CFU = Colony forming unit.
Remarks :
As per the results obtained (as mentioned in the above table ), it can be concluded that the above mentioned aire sterilizer when it is in operation (sterilizer was put on ), can reduce the bioburden of microbial (bacterial / fungal ) population to the extent of 90 % after two hours.
N.B. :
This report is issued with an explicit understanding that it would neither be used for the purpose of advertisement nor it would be produced as evidence in any form without the prior permission of the undersigned. further, this report is restricted to the sample submitted to the department only.
has been tested at the most reputed government laboratories for its efficacy in reducing micro-organisms
